Nuclear morphometric study of Non-Hodgkin's Lymphoma (NHL)
Journal: International Archives of Integrated Medicine (IAIM) (Vol.3, No. 5)Publication Date: 2016-05-15
Authors : Sridhar Reddy Erugula; P. Sujatha; Ayesha Sameera; B. Suresh Reddy; Jesudass Govada; G. Sudhakar; Kandukuri Mahesh Kumar;
Page : 58-63
Keywords : Non Hodgkin Lymphomas; Morphometry; Image analysis; Large Cell Lymphoma; Small Cell Lymphomas; Intermediate Cell Lymphomas; Nuclear features.;
Abstract
Background: Assessment of nuclear morphology is crucial for the diagnosis of non-Hodgkin's lymphoma. The non-Hodgkin’s lymphomas (NHL) constitute a heterogenous group of lymphoid neoplasms that vary in clinical behavior, histology, immunology and genetic profile. The diversity makes it difficult to diagnose accurately and reproducibly under light microscopy. The nuclear features play a major role in categorizing non-Hodgkin's lymphomas. Materials and methods: The present study was conducted for a period of two years in the Department of Oral Pathology, MNR dental College and Hospital, Sangareddy, Telangana, India. We encountered only three cases of Non-Hodgkin Lymphomas and so we have borrowed the some specimens of NHL from other hospitals in Hyderabad, India. Hematoxylin and Eosin (H and E) stained histological sections were selected and assessed for nuclear area, perimeter and nuclear size of 40 nuclei of tumour cells were measured using the Windows® based image analysis software Results: Total number of cases in our study was 15. The mean nuclear area, perimeter and nuclear size of neoplastic cells were studied. In our study, the mean nuclear area, perimeter and nuclear size of neoplastic cells were significantly high in large cell lymphomas as compared to intermediate cells and small cell lymphoma. P value was significant. Conclusion: Our study supports the assumption that nuclear morphometry offers a more objective and reproducible diagnostic method for subcategorizing lymphoid tumors than is currently possible by conventional histopathological techniques.
Other Latest Articles
- Analysis of common etiology between Takayasu’s Arteritis and Hyperthyroidism in a middle aged female in Eastern part of India
- A comparative study of oral manifestations of HIV among pediatric patients
- Evaluation of the sedative and anxiolytic effects of Midazolam and Clonidine as oral premedicant in paediatric patients undergoing elective surgery under general anaesthesia
- Effect of bupivacaine and bupivacaine-clonidine combination in supraclavicular brachial plexus block ? A comparative study
- Influence of various psychological correlates in cardiovascular disease patients
Last modified: 2016-06-01 21:52:08