Experimental Study of Releasing Nano Zinc Oxide from Wound Healing Biodegradable Polymer Film “Biodep-Nano”

Soft tissues pyo-inflammatory diseases and purulent processes are one of the most common complications of modern surgery. One of the main methods of topical treatment of these diseases is the use of polymer wound dressings acting in different phases of wound healing. In local treatment of wounds important issue is to achieve a therapeutic dose of available in polymer carrier drugs in the affected area as well as to preserve their action for the time being required for medical grounds, namely, prolongation of therapeutic effect. The objective of our research was to study the release of nano zinc oxide from wound healing biodegradable polymeric film “Biodep-nano” containing nano-particles. Materials and methods. We have developed a new tool for local treatment of wounds in the form of biodegradable polymer film “Biodep-nano” containing nano particles and saturated with nano zinc oxide. The research was carried out at the experimental bases of the Ivano-Frankivsk National Medical University, namely the laboratory “Center of Bioelementology” and the Department of General Surgery. The study of releasing active substances was performed using a colorimetric test system for precise and accurate determination of zinc in serum Aquaquant® with a sensitivity of 0.1-5 mg/l. manufactured by Merck KGaA (Germany). Results. Developed wound healing biodegradable polymer film gradually releases the active substance and nanoscale particles of nano zinc oxide from the proposed polymer composition faster than ordinary zinc oxide, which is confirmed by the experiment. Conclusions. 1. The applied methods allow us to study the release of nano oxide zinc and zinc oxide from wound healing biodegradable polymer films quantitatively. 2. Wound healing biodegradable polymer film “Biodep-nano” containing nano particles was experimentally found to be able to gradually release zinc ions into the area of injury for 2 days. 3. Nano zinc oxide releases from biodegradable film more quickly and in greater numbers than zinc oxide, which solves the problem of delivering an effective dose of the active ingredient to the affected area.