High Performance Liquid Chromatographic and Spectrophotometric Determination of Ranitidine HCl and Phenol in Injections
Journal: Austin Journal of Analytical and Pharmaceutical Chemistry (Vol.1, No. 5)Publication Date: 2014-11-05
Authors : Alaa El-Gindy; Mohammad Wafaa Nassar; Khalid Abdel-Salam Attia; Hamed Hamed Abu-Seada; Mahmoud El-Ghandour;
Page : 1-7
Keywords : Ranitidine HCl; Phenol; First-derivative spectrophotometry; First-derivative of the ratio spectra; HPLC;
Abstract
Two Spectrophotometric and HPLC methods are presented for the simultaneous determination of ranitidine HCl and phenol in pharmaceutical injections. The Spectrophotometric methods include first derivative (1D) ultraviolet spectrophotmetry with zero crossing measurement at 238.6 and 228.1 nm for ranitidine HCl and phenol, respectively and first derivative of the ratio spectra (1DD) with measurement of the amplitude of the peak to-trough at 323.2-328.1 nm and peak to zero at 280.1 nm for ranitidine HCl and phenol, respectively. The HPLC method was developed using C18 column with mobile phase consisting of 35 mM potassium dehydrogenate phosphate of pH 7- acetonitrile (78:28 v/v) with UV detection at 215 nm. The proposed methods were successfully applied for the determination of ranitidine HCl and phenol in synthetic mixtures and commercial injections.
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