Development and Validation of Novel Analytical Method for the Determination of Caroverine in Pharmaceutical Formulations

Caroverine is a spasmolytic drug but no monographs are available in official compendia for the analysis of this substance in raw and pharmaceutical dosage forms. The objective of this work was to develop a simple, fast and validated spectrophotometric method for routine analysis of caroverine in tablets. The newly proposed method depends on the charge-transfer complex formation between caroverine as n-electron donor and chloranil as p-acceptor to give a colored complex that absorbs maximally at 560 nm. Beer’s law is obeyed in the concentration ranges 2-30μg/ml with molar absorptivity of 2.8× 104 L mole-1 cm-1. The results of analysis of commercial formulations and the recovery study (standard addition method) of caroverine suggested that there is no interference from any excipients, which are present in pharmaceutical formulations. The proposed method is found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of caroverine bulk and commercially dosage form.