Development of HPTLC Method for the Estimation of Aceclofenac and Tolperisone Hydrochloride in Combination
Journal: Austin Journal of Analytical and Pharmaceutical Chemistry (Vol.2, No. 4)Publication Date: 2015-06-09
Authors : Patel DR; Mehta FA; Shah DA; Chhalotiya UK;
Page : 1-5
Keywords : HPTLC; Aceclofenac; Tolperisone Hydrochloride; Validation;
Abstract
A simple, sensitive, and precise high-performance thin-layer chromatographic method has been developed for estimation of Aceclofenac (ACF) and Tolperisone HCl (TOL) in combine dosage form. The method employs thin-layer chromatography (TLC) aluminum plates precoated with silica gel 60 F254 as the stationary phase, while the solvent system was n-butanol: 1, 4-dioxan(6:4v/v). The Rf values were observed to be 0.30 ± 0.02 and 0.60 ± 0.02 for ACF and TOL respectively. The separated band was densitometrically analyzed in absorbance mode at 268 nm. The method was linear in the range of 400-900 ng/band for ACF and 600-1350 ng/band for TOL. The limits of detection for ACF and TOL were found to be 14.41and81.54ng/band respectively. The limits of quantification for ACF and TOL were found to be 43.67 and 247.11 ng/band respectively. The proposed method was validated with respect to linearity, accuracy, precision and robustness.
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