Development and Validation of Analytical Method for Estimation of Memantine Hydrochloride

An accurate HPTLC analytical method for estimation of Memantine Hydrochloride was established and validated. Memantine Hydrochloride is an NMDA receptor antagonist and widely use for Alzheimer’s disease. The HPTLC method was developed using aluminum plates pre-coated with silica gel G60F254 as a stationary phase and n-Hexane: Ethyl acetate: Diethylamine (5:5:0.7 % v/v/v) as mobile phase. The separated spots were visualized as orange spots after dipping with Dragendorff’s reagent solution. The method was found to be Linear, Accurate, Precise, and Robust according to ICH Guideline. Linearity was found to be 5000-30000 ng/band for Memantine HCl. The LOD and LOQ were found to be 80.07ng/band and 242.637ng/band for Memantine HCl. So, developed method is applicable for the estimation of Memantine Hydrochloride.