GC-MS and HPLC Methods for Determination of Estriol Hormone in Pharmaceutical Preparations

This paper describes two rapid, sensitive and specific methods for determination of estriol hormone in pharmaceutical preparations by gas chromatography (GC) with mass spectrometry (MS) and high-performance liquid chromatography (HPLC) with fluorescence detection. The calibration curves were linear for estriol (r > 0.99) at the concentration range of 12.5- 500 ng/ml and 10-400 ng/ml for GC-MS and HPLC, respectively. The relative standard deviation (RSD) for intra- and inter-day precision was less than or equal to 4.72 and 6.25%, respectively. The developed methods were applied to a pharmaceutical preparation (Gynoflor) as tablet form which does not require any preliminary separation or treatment of the samples. No interference was found from tablet excipients at the selected assay conditions. Also, the results obtained from the methods were statistically compared and no significant difference was found.