A SIMPLE ASSAY METHODS DEVELOPMENT AND VALIDATION OF VALACYCLOVIR IN BLOOD, URINE AND PHARMACEUTICAL DOSAGE FORMS BY SPECTROPHOTOMETRY

Two rapid, simple, sensitive and selective spectrophotometric methods have been developed for the quantitative estimation of Valacyclovir hydrochloride in pharmaceutical formulations and different human body fluids (Blood and urine). The proposed methods were based on the reduction of the nitro group to amino group of the drug. The resulting amine was then subjected to proposed methods. Method A was based on the formation of oxidative coupling reaction between the corresponding drug and brucine ? NaIO4 to form violet colored chromogen at 543 nm. Method B was based on the oxidation followed by coupling with 3- methyl-2-benzothiazolinonehydrazone (MBTH) in presence of ferric chloride (FeCl3) to form green colored chromogen at 644 nm. The procedures described were applied successfully to the determination of the compound in their dosage forms and body fluids. Beer’s law is obeyed over the concentration ranges of 5?110 μg/mL and 3?45 μg/mL for brucine and MBTH, respectively, with correlation coefficients of 0.9968 and 0.9990, and molar absorptivity 0.4642× 104 and 0.9761 × 104 L/mol. cm for method A and method B, respectively. The analytical parameters such as apparent limits of detection (LOD) and quantification (LOQ) were also reported for two methods. Keywords: Spectrophotometry; Oxidation reaction; Valacyclovir hydrochloride; Pharmaceutical.