Genetic variability of broodstocks of restocking programs in Brazil
Journal: REVISTA MVZ CÓRDOBA (Vol.20, No. 3)Publication Date: 2015-09-01
Authors : Nelson Lopera-Barrero; Ed Lima; Luiz Filho; Elenice Goes; Pedro Castro; Aline Zardin; Angela Poveda-Parra; Ricardo Ribeiro;
Page : 4677-4687
Keywords : Genetic conservation; Leporinus elongatus; Pseudoplatystoma fasciatum; Rhamdia quelen; Salminus brasiliensis;
Abstract
Objective. The aim of this study was evaluate the genetic diversity of the following broodstocks: piapara (Leporinus elongatus), dourado (Salminus brasiliensis), jundiá (Rhamdia quelen) and cachara (Pseudoplatystoma fasciatum) already useful for restocking programs in the Paranapanema, Iguaçu and Paraná Brazilian Rivers. Materials and methods. Samples from the caudal fin of 122 fish were analyzed. DNA was extracted by NaCl protocol. PCR products were separated by a horizontal agarose gel electrophoresis. The fragments were visualized by staining with ethidium bromide. Results. The amplification of 25 primers generated different fragments in studied species that allowed characterizing 440 fragments of 100-2900 bp. High percentage of polymorphic fragments (66.67 to 86.29), Shannon index (0.365 to 0.486) and genetic diversity of Nei (0.248 to 0.331) were detected. Conclusions. The level of genetic variability in the broodstocks was adequate for allowing their use in restocking programs in the studied Rivers. However, periodical monitoring studies of genetic variability in these stocks, the mating system, reproductive system and general management must be made to guarantee the preservation of wild populations.
Other Latest Articles
- Environmental characterization of the reproductive season of migratory fish of the Sinú river (Córdoba, Colombia)
- Total mercury concentrations in fish from Urrá reservoir (Sinú river, Colombia). Six years of monitoring
- Estimation and comparison of conventional and genomic breeding values in Holstein cattle of Antioquia, Colombia
- Effects of Kikuyu grass (Pennisetum clandestinum) age and different forage: concentrate ratios on methanogenesis
- Concordance between otic cytology and culture in diagnosis of external otitis canine by Malassezia spp
Last modified: 2016-06-23 23:49:12