SERUM CORTISOL AND INSULIN HORMONE LEVELS AND THEIR ROLE IN NORTH INDIAN MEN AND WOMEN

Objective To study cortisol and insulin hormone serum levels and their role in north Indian men and women having Type 2 Diabetes. Research Design and Methods For the analyses, (n=200) subjects including (n=94) males and (n=106) females, out of which 100 diagnosed cases and 100 age and sex matched healthy controls were studied. Only diagnosed cases of diabetes type 2 (50 men and 50 women) aged 45?75 years undergoing glucose profile testing in outdoor clinics in the hospital PGIMS, Rohtak (2011-2013) were included following a detailed protocol. Patients with acute complications like coma and acidosis, pregnant women, postmenopausal women on hormone replacement therapy, use of steroids since past six months, type 1 diabetes were excluded. Early morning fasting samples were collected and serum analysed for cortisol, insulin, fasting blood glucose and HbA1c. Serum Insulin1,2 levels (normal healthy adults <25 ?lU/ml) were done using solid phase sandwich Enzyme linked immunosorbent assay, serum Cortisol levels3,4 (Reference range: 50-230 ng/ml) were done using DRG Cortisol ELISA kit -a solid phase enzyme linked immunosorbent assay, and HbA1c levels (normal=4-5.6% in normal people, <6.5% -target for control in diabetics) were measured on Auto analyser via Immunoassay Kits. The results were analysed and compared.