Population genetic structure of bovine lactoferrine gen in holstein breed of Antioquia Department
Journal: REVISTA MVZ CÓRDOBA (Vol.18, No. 1)Publication Date: 2013-01-01
Authors : Nancy Rodríguez C; Albeiro López H; Juli;
Page : 3355-3361
Keywords : Characterization; genetic analysis; polymorphism;
Abstract
Objective. Estimate some population structure parameters in a Holstein population of the Department of Antioquia. Materials and methods. Research involved 427 Holstein cows of five municipalities in the department of Antioquia. The cattle was genotyped using PCR-PFLP. Observed heterozygosity (Ho) and expected heterozygosity (He), the exact Hardy-Weinberg test (HW) and the structure and genetic differentiation among populations were calculated using F-Wright parameters which were assessed using the GENEPOP software. The allele and genotype frequencies were evaluated with the method described by Hartl. Results. The genotypic frequencies were 0.59, 0.37 y 0.04 to AA, AB y BB, respectively and allele frequencies were 0.78 and 0.22 of A and B. The population is in Hardy-Weinberg equilibrium. Heterozygosity was average among populations (Ho = 0.368). FIS, FST and FIT values of the total population were -0.0717, 0.0099 and -0.0611 but these were not significant. Conclusions. It was not possible to assume inbreeding or out breeding in the analyzed municipalities, except in the municipality of San Pedro de los Milagros, in which case the effect of genetic improvement and the reduction of heterozygosity was more strongly perceived.
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