USAGE OF PLATELET RICH PLASMA FOR STIMULATION OF COLONIZATION OF COLLAGENOUS MATRIXES BY HUMAN FIBROBLASTS

The procreation and grow power of fibroblasts of skin cadaver in vitro as a part of collagenic bandages, a dermic matrix and the spongeous demineralized bone, the platelets sated with components. It is shown that in vitro colonization the collagenous transplants by cells is the most effectively when using components of platelets after their activation or cryodestruction. The greatest growth rate of fibroblast is noted at concentration 150 pg (on 100 thousand cells) of platelet-derived growth factor, which receiving requires 100 million platelets with the granules revealed by means of intravital staining. In the presence of platelets material the intensity of fibroblast proliferation as a part of all types of collagenic transplants increases by 1.5-1.6 times without violation of cells structural integrity.