ANÁLISIS DE DOS POBLACIONES DE CANINOS (Canis familiaris) DE LA RAZA CIMARRÓN URUGUAYO UTILIZANDO RAPDs COMO MARCADORES MOLECULARES
Journal: REVISTA MVZ CÓRDOBA (Vol.13, No. 3)Publication Date: 2008-09-01
Authors : Silvia Llambí D; Rosa Gagliardi B; Mónica Martínez T; Jorge Estevez C; Ana Gorozurreta O; Gabriela Costa De F; Carolina Bianco Y; Rody Artigas S; María Victoria Arruga L;
Page : 1464-1468
Keywords : Canis familiaris; genome; Uruguay;
Abstract
Objective. To analyze the genetic variability in two populations of the native canine breed “Uruguayan Cimarron” from different areas of the country. Materials and methods. DNA was isolated from 40 animals of the Cimarron breed. Using PCR, 11 RAPD (random amplified polymorphism of DNA) molecular markers were analyzed. Results. 14.9% of loci were polymorphic. We found an index of shared bands of 0.91, a genetic distance of 0.16 and a Nei’s average diversity index of 0.08. Conclusions. Both populations present a high homogeneity and genetic identity for these markers suggesting that they belong to a common genetic nucleus.
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