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Using shf treatment microwave to obtain yeast cell wall polysaccharides

Journal: Food Science and Technology (Vol.10, No. 2)

Publication Date:

Authors : ; ; ;

Page : 7-13

Keywords : yeast Saccharomyces cerevisiae; polysaccharide; glucan; zymosan; microwave processing;

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Abstract

In the recent decades, a few polysaccharide types were isolated from yeast which are used as dietary supplements and even therapeutic medications. These include β-glucan and zymosan - a complex of β-glucan with mannoproteins. Beta-glucan has a broad spectrum of biological activity, such as immunomodulatory, antitumor, gastroprotective, detoxicating etc. There are different approaches to polysaccharides isolation from the cell wall of Saccharomycescerevisiae yeast. However, all these methods are typically time consuming, energy intensive and cannot allow achieving the satisfactory purity of the final prod-uct. In all cases, a mandatory phase is preliminary disintegration of the yeast cell wall. Efficiency of using for this purpose SHF irradiation of yeast mass suspended in the sodium hydroxide solution was estab-lished in the study. The preparations obtained by varying the treatment conditions were described. The possibility of obtaining yeast polysaccharides preparations of different composition using this method was shown. Both a monocomponent product β-glucan and a two-component complex of β-glucan and mannan (zymosan) can be isolated by varying NaOH concentration. The method is simple to apply, its use results in reduction of resource and energy consumption, improvement of environmental safety, which together de-termine the prospects of its use in the yeast polysaccharides technologies.

Last modified: 2016-08-17 06:01:54