Development of RP-HPLC Method for Estimation of Dithranol in Hydrogel based Lipid Nanoparticle Formulation
Journal: Current Trends in Technology and Sciences (Vol.1, No. 2)Publication Date: 2012-09-25
Authors : Sunil Prakash Chaturvedi Vimal Kumar;
Page : 104-107
Keywords : Psoriasis; Dithranol; RP-HPLC method; chromatogram; hydrogel.;
Abstract
A simple, specific, accurate, precise and reproducible method has been developed and validated for the estimation of Dithranol in lipid nanoparticle by RP-HPLC method. RP-HPLC estimation of dithranol in prepared formulation was performed using Phenomenex ODS 5μ, C18 column and Acetonitrile: Methanol: Buffer (20:20:60) as mobile phase which had shown sharp peak when detected at 258 nm. The linearity range was found to be in concentration range of 20-100 μg/mL for dithranol. The retention time, correlation coefficient and mean percentage recovery was 9.3 minutes, 0.9989 and 98.90 respectively. The % estimation of the drugs was found near to 100 % representing the accuracy of the method. Proposed method was validated for its precision, specificity, ruggedness and accuracy according to ICH guidelines. This method can be applied successfully in routine work for the determination of dithranol in prepared hydrogel based formulations
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