ISOLATION OF WEED DUAL TOPOPOISONS (I & II) FROM SOLANUM MAURITIANUM SCOP

The present research is focused on the phytochemical and biological investigation of Solanum mauritianum as a potent human dual topo I & II poisons. A good amount of secondary metabolites like alkaloids ( 163.10 ?g/g Atropine equivalents), phenolics (119.94 ug/g ?g/g Gallic acid equivalents), flavanoids (59.94 ?g/g quercetin equivalents) and saponins ( 14.2 % w/w) were identified from this plant. First time, a novel saponin glycoside Tetrahydro-2-(hydroxymethyl)-6-(octadecahydro-2,7,10a-trimethyl-1propylchrysen-8-yloxy)-2H-Pyran-3,4,5-triol (CH1) was isolated from the chloroform fraction of the aerial parts of this plant using flash chromatography, a total ESI-MS spectra was carried out in negative mode. The saponins namely, Nocturnoside ?b, Protodioscine, Paraquisoside ?b, Solanine, Solasodine and Solasonine were identified based on the mass peaks. The isolated compound CH1 also showed good CTC50 values on A- 549 (53.7±1.7), MCF-7 (64.6±1.2) & HCT-116 (72.8±0.6) incompare to standard Quercetin. Based on docking results on topoisomerase I ( 1K4T, 3AL2) and topoisomerase II (1ZXN, 3QX3) showed the apoptosis is may be due to dual topo- poisoning activity. The isolated compound (CH-1) arrest the cell cycle at S Phase and showed dual human topopoison I & II activity at 200 ?g/ml. Key words: Saponins, Flash chromatography, CTC50 Values , human dual topo I & II Poisons, saponins and Tetrahydro-2-(hydroxymethyl)- 6-(octadecahydro-2,7,10a-trimethyl-1propylchrysen-8-yloxy)-2H-Pyran-3,4,5-triol (CH1).