Bioanalytical Validated LC-MS Method for Determination of Naproxen in Human Plasma

Naproxen is an anti-inflammatory drug belongs to the category of analgesics and antipyretics. Naproxen has the ability to bind and inhibit the synthesis of prostaglandins and produces anti-inflammatory effect. Naproxen also inhibits COX-II which is involved in the inflammation. Bioanalytical method for naproxen has been developed using human plasma. Standard stock solution of naproxen was prepared using methanol. Zidovudine was used as internal standard. The linearity of the proposed method was developed in the range between 100 and 10000 ng/ml. The slope of the curve was found to be 111.46 and intercept was 6395.2 and correlation coefficient was 0.999. The proposed method was evaluated with validation parameters like accuracy, precision and stability. The developed method is suitable for pharmacokinetic studies.