Effect of Cytokinin and Auxin on Callus Formation and Shoot Multiplication of Strawberry (Fragaria x ananassa Duch.) under in vitro Condition

The experiment was pursued in Tissue Culture Laboratory of Department of Horticulture in Sardar Vallabhbhai Patel University of Agriculture & Technology Meerut during 2015-16 on Chandler variety of strawberry. N6 media were prepared. Maximum callus formation in mature leaf explants (81%) was noted under the treatment of BAP 2mgl?1 combined with IBA 1.0 mgl?1. Maximum callus formation in young leaf (74.0%) was noted under the treatment of BAP 2mgl?1 combined with IBA 1.0mgl?1.Maximum callus induction in internodes (47.6%) was noted under the treatment of BAP 2mgl?1 combined with IBA 1.0 mgl?1 . Highest number of shoots (14.00) from mature derived callus at four weeks after inoculation were noted under the treatment of BAP 2mgl?1+ Kinetin 1.5 mgl?1. Maximum number of shoots (11.66) from young leaf derived callus of strawberry cv. Chandler at four weeks after inoculation were noted under BAP 2mgl?1 combined with Kinetin 1.5 mgl?1 and BAP 3mgl?1 alone. The highest number of shoots (10.33) from internodes derived callus at four weeks after inoculation were noted with BAP 3mgl?1 alone. Viewing above observations it is concluded that BAP 2 mgl?1 + IBA 1.5 mgl?1 and Kinetin 1.5 mgl?1 + IBA 1.0mgl?1 showed better performance on accordance of callus formation in mature leaf, young leaf as well as internodes. BAP 2 mgl?1 + Kinetin 2mgl?1 showed better performance on accordance of shoot induction in mature leaf, young leaf as well as internodes.