Effect of Cytokinin and Auxin on Callus Formation and Shoot Multiplication of Strawberry (Fragaria x ananassa Duch.) under in vitro Condition
Journal: HortFlora Research Spectrum (Vol.5, No. 3)Publication Date: 2016-09-28
Authors : Yogesh Prasad Rajbhar; Sumit Tomar; Hariom Katiyar; Mukesh Kumar; Anil Kumar; Govind Rajbhar;
Page : 206-212
Keywords : Strawberry; In-vitro; Shoot Multiplication; Cytikinin; Auxin; Callus Formation;
Abstract
The experiment was pursued in Tissue Culture Laboratory of Department of Horticulture in Sardar Vallabhbhai Patel University of Agriculture & Technology Meerut during 2015-16 on Chandler variety of strawberry. N6 media were prepared. Maximum callus formation in mature leaf explants (81%) was noted under the treatment of BAP 2mgl?1 combined with IBA 1.0 mgl?1. Maximum callus formation in young leaf (74.0%) was noted under the treatment of BAP 2mgl?1 combined with IBA 1.0mgl?1.Maximum callus induction in internodes (47.6%) was noted under the treatment of BAP 2mgl?1 combined with IBA 1.0 mgl?1 . Highest number of shoots (14.00) from mature derived callus at four weeks after inoculation were noted under the treatment of BAP 2mgl?1+ Kinetin 1.5 mgl?1. Maximum number of shoots (11.66) from young leaf derived callus of strawberry cv. Chandler at four weeks after inoculation were noted under BAP 2mgl?1 combined with Kinetin 1.5 mgl?1 and BAP 3mgl?1 alone. The highest number of shoots (10.33) from internodes derived callus at four weeks after inoculation were noted with BAP 3mgl?1 alone. Viewing above observations it is concluded that BAP 2 mgl?1 + IBA 1.5 mgl?1 and Kinetin 1.5 mgl?1 + IBA 1.0mgl?1 showed better performance on accordance of callus formation in mature leaf, young leaf as well as internodes. BAP 2 mgl?1 + Kinetin 2mgl?1 showed better performance on accordance of shoot induction in mature leaf, young leaf as well as internodes.
Other Latest Articles
- Nutritional Status of Malta Orchards in Bikaner District
- Effect of Silicon Bunch Spraying and Bunch Bagging on Yield, Quality and Shelf Life of Banana var. Grand Naine
- Evaluation of Chrysanthemum (Chrysanthemum morifolium Ramat) Genotypes under West Garo Hills District, Meghalaya
- Character Association and Path Co-efficient Analysis in Garlic (Allium sativum L.)
- Leafing, Flowering and Fruiting of Sterculia setigera in Metema, North Western Ethiopia
Last modified: 2016-10-27 23:44:52