Experimental Study on Human Demineralized Dentin Matrix as rhBMP-2 Carrier In Vivo

Purpose: This study examined the bone induction capacity of human demineralized dentin matrix as an rhBMP-2 carrier. Method: Total 40 nude mice weighing 15-20g were used for the study. Two types of scaffold were selected as a carrier of rhBMP-2: Inorganic bovine bones, tricalcium phosphate (TCP) as the control group where Autogenous tooth bone grafts material (AutoBT), a human demineralized dentin matrix (DDM), as the experimental group. Laboratory animals were sacrificed at two and four weeks after the implanting two rhBMP-2 carriers into intramuscular pouch. The evaluation of the specimen was divided into four compartments: field phenomenon, cell number (osteoblast, firbroblast), osteoid formation, and maturation, and compared two carriers histologically. Result: Field phenomenon: Both groups have excellent fibrous encapsulations around particles; Cell number (osteoblast, fibroblast): The early cellular reaction on the surface of particles was superior in the DDM group; Osteoid formation: more osteoid was deposited on the DDM group; Maturation: The DDM group showed compact osteoid bridges and dense fibrous connective tissue filled between each particle, but the TCP group showed that the ostoid deposited seemed to be transformed into fatty tissues after 4 weeks. Conclusion: Both the DDM/rhBMP-2 and TCP/rhBMP-2 induced bone in intramuscular pouch of nude mice, but more bone was formed in DDM/rhBMP-2 group.