INFLUENCE OF SURFACE-ACTIVE RHAMNOLIPID BIOCOMPLEX AND ETHYLTHIOSULFANILATE ON GROWTH AND BIOCHEMICAL PARAMETERS OF PLANTS IN THE OIL CONTAMINATED SOIL

The aim of the work was to study the influence of rhamnolipid biocomplex and ethylthiosulfanilate on field pea and sorghum plants when growing in petroleum contaminated soil. Plant seeds were treated with solutions of rhamnolipid biocomplex or ethylthiosulfanilate (0.01 g/l) before planting and grown in containers with soil artificially contaminated with petroleum (5, 8 and 10%). Effect of rhamnolipid biocomplex and ethylthiosulfanilate was determined by the determination of growth (weight, length plants) and biochemical parameters (content of photosynthetic pigments, hydrogen peroxide, malondialdehyde). The stimulating effect of rhamnolipid biocomplex and ethylthiosulfanilate on growth parameters of field pea was shown: the shoot mass has significantly increased on 39%, root mass ? on 26% if compared with the control. For sorghum somewhat smaller increase of growth parameters was observed. Under the influence of rhamnolipid biocomplex and ethylthiosulfanilate the content of photosynthetic pigments in field pea and sorghum has also increased. It was determined that the action rhamnolipid biocomplex and ethylthiosulfanilate promoted the decrease of indicators of plant oxidative reactions if compared with control: the content of hydrogen peroxide ? in average on 15% and 16%, malondialdehyde ? on 13.5% and 16% respectively. The results of the study testify to the effectiveness of rhamnolipid biocomplex and ethylthiosulfanilate as growth stimulators for field pea and sorghum, as well as improvement of adaptive capability of plants to unfavorable conditions. It creates the prospects of their application as effective and ecologically safe substances for the intensification of contaminated soil phytoremediation.