OBTAINING OF RECOMBINANT ANALOGUE OF HUMAN SHB-EGF USING OF TRANSPOSON-TRANSPOSASE GENETIC SYSTEM

The goal of work was obtaining of recombinant soluble form of heparinbinding EGF-like growth factor sHB-EGF in eukaryotic cells based on transposon genetic system. We used methods of recombinant DNA and obtaining of eukaryotic cells for producing of recombinant proteins. We have obtained stable eukaryotic producer of recombinant analogue of soluble form of human heparinbinding EGF-like growth factor sHB-EGF based on HEK293 cells. We used transposon based non-viral expression system for sHBEGF expression. Moreover, studies in mice have shown the possibility of using sHB-EGF expressing genetic constructs for in vivo gene therapy of various pathological conditions.