MILK-COAGULATION AND PROTEOLYTIC ACTIVITY OF ?GLYECK? ? CARPATHIAN ENZYME PREPARATION
Journal: Biotechnologia Acta (Vol.8, No. 2)Publication Date: 2015-04-30
Authors : A.V. Iukalo;
Page : 91-95
Keywords : milk-clotting preparation ?Glyeck?; milk-clotting activity; proteolytic activity; casein fractions.;
Abstract
The purpose of this work was to study milk-clotting and proteolytic properties of preparation ?Glyeck?. Using Soxhlet method we showed that the preparation possesses a high milk-clotting activity, and this activity doesn't change a lot during the storing process (up to 2 years). This is due to two processes taking place simultaneously: decrease in milk-clotting activity of the preparation and reducing the water content in the preparation. Proteolytic activity of the preparation was investigated by both: the modified Benki method and electrophoresis in polyacrylamide gel alkaline system. Electrophoretically homogeneous fractions of αS1-, β-, and k-caseins were obtained to characterize the specificity of the proteolysis. The specificity of cleavage of casein fractions by ?Glyeck? preparation was compared with this one of the rennet enzyme and pepsin. It was found that ?Glyeck?, rennet enzyme and pepsin actively split k -casein. Preparation ?Glyeck? showed low proteolytic activity to αS1- and β -caseins that is typical for purified chymosin. Electrophoretic analysis of proteolysis products of casein fractions confirms the high specificity of ?Glyeck? preparation towards k -casein. Casein fractions such as αS1- and β -casein were more resistant to this preparation. The results indicate that preparation ?Glyeck? is a natural milk-clotting preparation with high quality and it is mainly consists of protelytic enzyme chymosin.
Other Latest Articles
- Bacillus amyloliquefaciens SUBSP. plantarum PROBIOTIC STRAINS AS PROTEASE PRODUCERS
- THE OVEREXPRESSION OF GENE ENCODING RICE POTASSIUM CHANNEL - OsТРКa INCREASES THE SALT AND DROUGHT TOLERANCE OF PLANTS
- In vitro SELECTION OF WINTER TRITICALE FOR THE RESISTANCE TO WATER DEFICIT
- BIOLOGICALLY ACTIVE SUBSTANCES OF METHANOL EXTRACTS OF DRIED Lactarius quetus AND Lactarius volemus BASIDIOMES MUSHROOMS: IDENTIFICATION AND POTENTIAL FUNCTION
- COMPARATIVE MOLECULAR GENETIC ANALYSIS BETWEEN UKRAINIAN AND EU REGISTERED GLYPHOSATE-TOLERANT RAPESEED TRANSGENIC PLANTS
Last modified: 2016-11-12 01:49:45