OPTIMIZATION OF TANNASE POSITIVE PROBIOTIC PRODUCTION BY SURFACE RESPONSE METHODOLOGY
Journal: Biotechnologia Acta (Vol.7, No. 5)Publication Date: 2014-10-30
Authors : Yumnam S.; Prasanna B.; Oriabinska L. B.; Khrokalo L. A.; Dugan O. M.;
Page : 62-70
Keywords : Lactobacillus; optimization of medium; probiotic; response surface methodology.;
Abstract
Study in conditions in vitro of eight Lactobacillus strains procured from culture repositories for their probiotic potential and extracellular tannase activity was the aim of the research. Based upon acid, bile salt tolerance and antibiotic resistance L. plantarum MTCC 2621 with high tannase activity was selected for production studies. Optimization of nutrient medium in 3 L bioreactor was optimized by Surface Response Methodology based on the Full Factorial Central Composite Design. A factorial design 23 augmented by 6 axial points (α = 1.68) and six replicates at the center point was implemented in 20 experiments. The optimized conditions were found to be pH 5.69, contain of lactose 128.58 g/l, peptone 8 g/l. A tenfold increase in the biomass production was observed using the optimized nutrient medium in bioreactor as compared to initial MRS medium.
Other Latest Articles
- OBTAINING AND CHARACTERISTIC OF CURCUMIN LIPOSOMAL FORM
- Insight to Computational Linguistics
- TASK OF EPANET ANALYSIS OF EXISTING WATER DISTRIBUTION SYSTEM AT DIRE DAWA CITY, ETHIOPIA
- RESISTANCE OF KARST CAVERNS NITROGEN-FIXING BACTERIA TO EXTREME FACTORS
- Non Linear Analysis of Two lobe Journal Bearings with Surface roughness
Last modified: 2016-11-13 22:05:48