THE MUTANT STRAINS OF MICROORGANISMS ? PRODUCERS OF LYSINE AND THREONINE

Strains-producers of essential amino acids of aspartate family such as Corynebacterium glutamicum, Brevibacterium flavum, Brevibacterium sp. 90, Brevibacterium sp. 90H, Brevibacterium sp. E531 from ?Collections strains and lines of plants for food and agricultural biotechnology? of ?Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine? for biosynthetic activity for lysine and threonine were investigated. Active strains-producers of amino acids were obtained after UV irradiation, biological characteristics of these organisms were studied and their biosynthetic efficiency was estimated. New mutant strains of threonine and lysine were selected using analysis of regulatory and analogorezistent auxotrophy. Sensitivity of output and mutant strain-producers to penicillins, macrolides, cephalosporins, tetracyclines, and other groups of antibiotics was investigated. Biosynthetic activity of obtained threonine producers ? Brevibacterium flavum IMВ B-7446 and lysine ? Brevibacterium sp. IMВ B-7447 on the production of target amino acids was determined. Strains are deposited in the ?National Depository microorganisms? of the Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine.