CYTOTOXIC AND DNA-DAMAGE ACTIVITY OF THE PREBIOTIC SUBSTANCES

Due to the widespread of the ecological disorders and diseases of the gastrointestinal tract of humans and animals there is an increased interest in probiotic products (probiotics, prebiotics and synbiotic), which showed its efficacy and safety in their treatment and prevention. Yeast Saccharomyces cerevisiae and cell culture of a human larynx cancer НЕр-2 were used to study cyto- and DNA-damage effects of several polysaccharides (arabinogalactan and nanobiocomposite of arabinogalactan with flavonoids, karraginan, galactomannan and their hydrolyzed derivatives), which are new perspective prebiotic substances. Cytotoxic potential of substances was determined from MTT-assay and DNA-damage effects were determined by morphological and structural features of cell nuclei. The synergic action of the polysaccharides with chemical triggers of cellular damages (hydrogen peroxide, acetic acid, nitrosomethylurea) was evaluated. The studied substances possessed no cyto- and DNA-damage action on the yeast and mammalian cells. It was shown that polysaccharides were able to enhance the effects of hydrogen peroxide, acetic acid and nitrosomethylurea. Nature of the marked effects remains unclear, but it may be assumed that the studied prebiotic substances can be used to enhance the effects of some chemical compounds and appear promising for use in integrated biomedical preparations of varied spectrum.