FLUORESCENT PROBES FOR INVESTIGATION PEPTIDE NATURE SUBSTANCES IN THE CRYOPRESERVED ORGANS FRAGMENTS EXTRACTS

The work aimed to investigate the use of new probes Е-176, К8-3000 and К8-1300 to research the specificity of peptides in the extracts of cryopreserved fragments of organs of pigs' and piglets' and their interaction with blood serum albumin. Extracts of cryopreserved fragments of skin of new-born piglets', spleen of pigs', heart of mature pigs' and new-born piglets' cleared of proteins were used in the research. Titration of extracts with investigated probes revealed differences in the peptides properties that are a part of them, to bind probes, reflecting tissue specificity of peptide drugs. Adding the extracts to albumin solution triggered quenching of intrinsic fluorescence of albumin and affected the state of tryptophan residues of the protein. Investigated probes show a high sensitivity to the interaction with blood serum albumin substances of peptide nature and could be recommended as new fluorescent probes for use in the biomedical researches. Since fluorescence of these probes is excited in the long wavelength of spectrum, it enables to reduce the artifacts by their use that are introduced by chromophore groups of the biomacromolecules absorbing in the visible region.