Enhanced Lipase Production by Solid State Fermentation using Mustard Oil cake as Substrate

The most productive strain, identified as Bacillus subtilis ? OCS ? 02 was cultivated for lipase production in solid state fermentation (SSF) using oilcake residue as a solid support. Solid-state fermentation (SSF) was carried out using mustard oil cake (MOC) as substrate for the production of lipase. Solid waste from the mustard oil industry was used as the basic nutrient source and was supplemented with peptone, olive oil or starch at different C/N ratios. Different parameters such as substrate selection, p H of the medium, incubation time, effect of moisture content of substrate were optimized. The highest lipase activity (15.4 U/g initial dry weights) was achieved after 48 hours of fermentation. The enzyme production was growthrelated, the activity being the maximum when the bacterial biomass was at its peak at 24 h.