ACTIVITY DEPENDENCE OF CELLULOLYTIC ENZYMES MONOAND DIKARYOTIC CULTURES OF Stereum hirsutum (Wild.) Gray (Basidiomycetes) FROM A SUBSTRATUM
Journal: Biotechnologia Acta (Vol.6, No. 3)Publication Date: 2013-06-30
Authors : S. M. Boiko; M. E. Ryazanova;
Page : 116-120
Keywords : cellulolytic enzymes; Stereum hirsutum; monokaryotic cultures; electrophoresis of proteins.;
- ACTIVITY DEPENDENCE OF CELLULOLYTIC ENZYMES MONOAND DIKARYOTIC CULTURES OF Stereum hirsutum (Wild.) Gray (Basidiomycetes) FROM A SUBSTRATUM
- DONOR VALUE OF THE NEW WILD AND RUDERAL SUBVARIETIES OF G. HIRSUTUM L. FROM MEXICO
- Improvement of fungal cellulolytic and xylanolytic enzymes production by new formulation of culture medium using wastes
- STUDY OF THE DONOR VALUE OF THE SAMPLES IN NEW WILD AND RUDERAL VARIETIES OF G.HIRSUTUM L., USED IN THE HYBRIDIZATION
- Saccharification of Alkaline Treated Rice Straw by Subsequently Hydrolysis of Xylanolytic-Cellulolytic Enzymes for Xylooligosaccharides and Glucose Production
Abstract
Activity of cellulolytic enzymes cultures Stereum hirsutum (Wild.) Gray, different nuclear status, depending on the substrate have been studied. The most productive by synthesis endoglucanases monokaryotic cultures of Sh1-4 and Sh7-2 were determined. It is found that nutrient medium containing filtering paper is optimal for synthesis of cellulase (culture Sh1-4, maximum of cellulolytic activity is 319 ?М/mg, culture Sh7-2 ? 101 ?М/mg). Maximum of cellulolytic activity at cultivation with sawdust of trees for culture Sh1-4 observed at the 10th day was 142 ?М/mg (oak sawdust), for culture Sh7-2 at the 20th days it was 34 ?М/mg (ash-tree sawdust). Study of quality cellulolytic enzymes enabled to establishe presence of four exoenzymes of Cx complex with a relative electrophoretic mobility of 0.22; 0.24; 0.47; 0.74. Enzyme with relative electrophoretic mobility 0.47 showed high concentration and activity.
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