THE FREE PROLINE CONTENTS OF THE SUNFLOWER TISSUES THROUGHOUT THE MORPHOGENESIS in vitro

Low frequency of in vitro plant regeneration limits significantly wide expansion of biotechnology methods. The screening of explants with stable and high level of the morphogenesis is an open problem. It is known, that free proline fluctuations correlate with processes of plant growth and development in vivo and in vitro. Sunflower mature seeds were germinated in vitro. The contents of free proline at somatic tissues with different regenerational abilities were measured. Following explants were estimated: cotyledons from 1- or 4-days cultivating shoots; primary explants prepared for the regeneration procedure (segments of 4-day cultivating shoots); primary explants with and without initial morphogenesis, 6 days on the regeneration medium; shoots and certain explants at the moment of their mutual separation; explants, 10 days after shoots release. The top levels of free proline were observed in tissues with fulfiled organogenesis. The hypothesis about proline involvement to the process of sunflower morphogenesis in vitro is discussed. During primary screenings of explants the level of free proline can be appropriate index of in vitro sunflower organogenesis.