PRELIMINARY DATA ON EXTRACTION, ISOLATION AND CHEMICAL ANALYSIS OF VOLATILE COMPOUND OF TUBERS OF GYMNOSPERMUM ALTAICUM SUBSP. SCIPETARUM WITH DICHLORMETHANE

Recently Albanian biologist discovered a new plant unknown before in Albania. The DNA analysis showed that this is a species of Gymnospermium altaicum scipetarum (Tan K., Mullaj A.,2001) that grows mainly in Albania(Mayer, E.,1983). For this plant there are proofs that grow even in Montenegro, Rumija Mountain (Green P., Tutin G., Heywood H., Burge A..,1972). The name Scipetarum was proposed by Albanian biologists but is not registered yet. In our laboratory we were interested in screening the chemical composition of tubers of this plant. In this paper we will present the preliminary results on the analysis of the tubers of this plant extracted with dichloromethane (DCM). The extract obtained was analyzed by thin layer chromatography and gas chromatography. Based on the large variety of compounds that were extracted was necessary to use separation with column chromatography and then the identification of these compounds with mass-spectroscopy. The analysis and identification was made with GC-MS. Are identified some triterpenoids like squalene, different derivatives of stigmasta, Cholest-5-en-3-ol and some alcohols, alkanes, alkenes and esters. For full identification is needed further work with different techniques like NMR or MSMS identification.