THE PHOSPHATIDYLETHANOLAMINE DERIVATIVE diDCP - LA - PE STIMULATES VESIVULAR GLUT4 EXOCYTOSIS BY INTERACTING WITH NSF

We have earlier found that the phosphatidylethanolamine derivative ,1,2 - O - bis - [8 - {2 - (2 - pentyl - cyclopropylmethyl) - cyclopropyl} - octanoyl] - sn - glycero - 3 phosphatidylethanolamine (diDCP - LA - PE) promotes translocation of the glucose transporter GLUT4 towards the cell surface and facilitates glucose uptake into cells. The presen t study was conducted to understand the role of N - ethylmaleimide - sensitive factor (NSF) in the diDCP - LA - PE - induced GLUT4 trafficking. Like insulin, diDCP - LA - PE increased cell surface localization of GLUT4 in differentiated 3T3 - L1 adipocytes, and the effe ct was abolished by the vesicular exocytosis inhibitorbotulinum toxin - A. diDCP - LA - PE - induced GLUT4 translocation is clearly inhibited by knocking - down NSF. These results indicate that diDCP - LA - PE stimulates vesicular GLUT4 exocytosis by interacting with NSF.