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Preliminary Characterization of a NADPH Dependent Chromate Reductase from Trichoderma pseudokoningii

Journal: Journal of Microbiology & Microbial Technology (Vol.1, No. 1)

Publication Date:

Authors : ; ; ;

Page : 01-04

Keywords : Trichoderma pseudokoningii; Chromate reductase; Hexavalent chromium; Purification;

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Abstract

Intracellular chromate reductase enzyme extracted from a chromium tolerant strain Trichoderma pseudokoningii was partially purified by ultrafiltration and gel filtration chromatography. The purified fraction showed highest reducing activity in presence of 1% (w/v) hexavalent Chromium. NADPH at a conentration of 2 mM was found to act as electron donor in the reduction reaction. The intracellular chromate reductase was found to have an optimum pH and temperature of 7.4 and 70 °C respectively. The enhancement of reductase activity in presence of exogenous thiols indicated the presence of thiols at active site.

Last modified: 2016-12-12 14:59:24