Preliminary Characterization of a NADPH Dependent Chromate Reductase from Trichoderma pseudokoningii
Journal: Journal of Microbiology & Microbial Technology (Vol.1, No. 1)Publication Date: 2016-06-30
Authors : Rina Rani Ray; Debpali Sur; Aditi Kundu;
Page : 01-04
Keywords : Trichoderma pseudokoningii; Chromate reductase; Hexavalent chromium; Purification;
Abstract
Intracellular chromate reductase enzyme extracted from a chromium tolerant strain Trichoderma pseudokoningii was partially purified by ultrafiltration and gel filtration chromatography. The purified fraction showed highest reducing activity in presence of 1% (w/v) hexavalent Chromium. NADPH at a conentration of 2 mM was found to act as electron donor in the reduction reaction. The intracellular chromate reductase was found to have an optimum pH and temperature of 7.4 and 70 °C respectively. The enhancement of reductase activity in presence of exogenous thiols indicated the presence of thiols at active site.
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