HPLC Analysis of Flavonoids in Achyranthes aspera and Cissus quadrangularis

Reversed phase high performance liquid chromatography (RP-HPLC) with UV detector was done for the analysis of four naturally occurring flavonoids of Achyranthes aspera and Cissus quadrangularis namely lutioline, myricetin, quercetin, kaempferol. The separation of filtered methanolic extracts of selected plants as well as a mixture of authentic standard flavonoid samples of lutioline, myricetin, quercetin, kaempferol was done within 35 min. using an RP C18 column, at ambient temperature, running conditions included: injection volume 20?l; mobile phase: methanol: water (1:1) (0-10 and (7:3) (10-20 min), flow rate 1 ml/min; and detection at 339nm. Identifications of specific flavonoids are made by comparing their retention times with those of the standards. Three flavonoids in Achyranthes aspera and two flavonoids in Cissus quadrangularis were detected by HPLC.