FORMULATION, CHARACTERIZATION AND IN-VITRO EVALUATION OF NANOSUSPENSION FORMULATION OF CLOPIDOGREL USING SOLVENT-ANTISOLVENT TECHNIQUE

Aim: The main aim of our study was to improve solubility of Clopidogrel bisulphate by preparing nanosuspensions using solvent-antisolvent (bottom up) technology. Methods: Clopidogrel nanosuspensions were formed by solvent antisolvent method. 15 formulations were prepared with different concentrations at different ratios. These formulations were evaluated for mean particle size, solubility, drug content and maximum yield. The selected formulation was then compared with pure drug for various parameters such as X-ray diffraction, Scanning Electron Microscopy, in-vitro Dissolution studies, Fourier Transform ? Infrared Radiation (FT-IR) etc. Release kinetics and stability studies were performed for the optimized formulation. Results: Out of 15 formulations, F15 comply well with all the parameters. On comparison with pure drug, F15 showed better characteristics such as Fourier Transform- Infrared Radiation (FT-IR), Solubility, particle size, Scanning electron microscopy, in-vitro dissolution, X-ray diffraction etc. Optimized formulation showed first order kinetics and stability was shown for over 3 months. Conclusion: Clopidogrel (anti-platelet) in nanosuspension formulation can overcome the limitation of low solubility, dissolution, bioavailability and explore further. Keywords: Clopidogrel, Nanosuspension, Solvent-antisolvent, Bioavailability, Release Kinetics