Comparison of SNP Polymorphism of Alcohol Metabolizing Related Enzyme Genes in Intoxicated Individuals and Nonalcoholic Population

Objective: To compare the distributions of SNP allele and genotype about alcohol dehydrogenase 2 (ADH2), aldehyde dehydrogenase 2 (ALDH2) and CYP2E1 in intoxicated individuals and nonalcoholic population. Methods: 100 individuals who were penalized duo to intoxicated driving were genotyped for 40 SNPs of ADH2,ALDH2 and CYP2E1 using multiplex PCR and Iplex chemistry on a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer and compared with a reference group of 99 blood donors. Allele frequencies and genotype frequencies of 40 SNP loci were calculated and compared in the two groups. Results: Among the 40 SNP loci, seven SNPs (e.g. rs698, rs2241894, rs1789915, rs13306164, rs671, rs28371746 and rs2515641) were polymorphic in the intoxicated population, but only six SNPs (e.g. rs698, rs2241894, rs13306164, rs671, rs28371746 and rs2515641) were polymorphic in the control individuals. Two SNP loci (rs671 in and rs2515641) were found to have a significant difference in frequency distribution between the two populations (p<0.01). Conclusion: Among the above 40 SNP loci of ADH2,ALDH2 and CYP2E1 genes, rs671 and rs2515641 in the intoxicated population were found to differ in allele and genotype frequency distribution from the nonalcoholic population and may be related to alcohol intoxication.