Interpretation: Real Time Assessment on Immotile but Viable Spermatozoa for Intracytoplasmic Sperm Injection (Icsi): an Embryologists Outlook

Material and Method: To help in selection, quite a few techniques have been developed to recognize viable spermatozoa from the immotile portion. Amongst the foremost well-known used approaches are: (i) The hypo-osmotic swelling test (ii) Modified hypo osmotic swelling test (iii) Chemical compound for initiation of tail actions (iv) The sperm tail flexibility test (v) Laser-assisted immotile sperm selection (vi) Birefringence-polarization microscopy and (vii) Raman micro spectroscopy***. Interpretation: A healthy and viable spermatozoon is the most importantly required for commencement of fertilization development and progression in ICSI. Usually motility is the crucial and principal sign used to conclude a sperm's viability. Nevertheless, in every Assisted Reproduction Technology (ART) procedure likely In Vitro Fertilization (IVF) assisted conception units cases are experienced in which not an iota or only little spermatozoa are motile. This inevitable dilemma can occur in treatment cycles (couple undergoing treatment) entailing ejaculated samples but, is utmost frequent and general in cases where surgically extracted testicular spermatozoa are retrieved. Conclusion: All this can be used routinely in an IVF laboratory with each having both potencies and limitations. The purpose of this petite review is to spot light on the technical concern involved in the routine of each of these techniques and to focus the probenefits and drawbacks of each one approach.