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Lipase Production from Bacillus subtilis using various Agricultural waste

Journal: International Journal of Advanced engineering, Management and Science (Vol.3, No. 5)

Publication Date:

Authors : ; ; ; ; ;

Page : 405-409

Keywords : Lipase; Bacillus subtilis; Surface Fermentation; agricultural wastes; optimization.;

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Lipases was produced by Bacillus subtilis PCSIR NL-38 strain and rape seed oil cake as substrate. Surface fermentation of minimal media in 250ml conical flask under static conditions gave 12.81 U/ml of lipases at 40°C for 48 hours. Lipase activity was monitored titrimatrically. Optimization of physicochemical parameters indicated that PCSIR NL-38 showed maximum lipase production at pH 7 with NH4NO3 as inorganic nitrogen source, glucose as carbon source, FeSO4.7H2O as salt, with 7% inoculum size and 96 hours of incubation.

Last modified: 2017-05-09 02:09:44