EVALUATION OF ANTIDIABETIC ACTIVITY OF LEAVES EXTRACT OF POPULUS DELTOIDES

Aim: The study was designed to evaluate the Antidiabetic activity of ethanolic leaves extract of P. deltoides. Method: The P. deltoides ethanolic leaves extract was tested for the presence of various phytoconstituents using standard procedure. The leaves extract was tested for its hypoglycaemic property in healthy albino rats and glucose loaded rats. The effect of extract was evaluated for its antidiabetic action in alloxan induced rats. The effect of extract on heart and kidney was determined by measuring biomarkers like total cholesterol, triglycerides, HDL, creatinine, BUN, CPK and protein in diabetic rats. The histopathology of pancreas was studied to support the experimental results. Result: The P. deltoides ethanolic leaves extract revealed the presence of variety of chemical constituents like glycosides, tannins, amino acids, alkaloids, protein, flavanoids, terpenes, phenols, saponins. The P. deltoides leaves extract exhibited significant reduction in blood glucose level in healthy albino rats and overloaded glucose levels. The extract exhibited significant reduction in blood glucose level in alloxan induced diabetic rats. The percentage of reduction was highest at 500 mg/kg dose of ethanolic extract at 48.55% at 7th day of the treatment. The extract protected experimental animals against cardiotoxicity and nephrotoxicity in diabetic and normal animals. Conclusion: The ethanolic extract of P. deltoides exhibited significant hypoglycaemic property in healthy and diabetic albino rats. The extract protected the diabetic albino rats against cardiac and nephrotoxicity. Keywords: alloxan, cardioprotective, hypoglycemia , Populus deltoides