Analytical method development and validation of Mirabegraon by RP-HPLC Method

A novel very rapid, sensitive, reverse phase High Performance Liquid Chromatography (RP-HPLC) technique was developed for the quantitative estimation of mirabegraon in bulk and tablet dosage form..It was resolved by using a mobile phase Potassium di-hydrogen phosphate: acetone in the ratio (40:60 v/v) at a flow rate of 1.0 mL/min. using UV - Visible detector at the wavelength of 243 nm for quantification. Efficient separation was achieved for mirabegraon on used Waters Acquity HSS C18(100 × 2.1 mm, 1.7ìm). The retention time mirabegraon of was 2.754min. The calibration graphs were linear and the method showed excellent recovery for tablet dosage form. The developed method was validated according to the International Conference on Harmonization (ICH) guidelines with respect to linearity, accuracy, precision, specificity and robustness.