DETERMINATION OF ETRAVIRINE IN HUMAN PLASMA BY HPLC

A simple bioanalytical HPLC method for the estimation of Etravirine in human plasma has been developed and validated. Fenofibrate was used as an Internal Standard . Extracted sample was eluted using C18 (250 x 4.6 mm, 5 μm) column. The mobile phase consisted of Acetonitrile:Water in the ratio of 85:15 v/v which was sonicated to degas and delivered at a flow rate of 1 ml/min at ambient temperature. The retention time of Etravirine and Fenofibrate was 5.32(± 0.1) and 3.58(± 0.1) minutes respectively. Studies were performed using an HPLC system equipped with a UV detector; the response was monitored at 308 nm. The method was validated according to MHLW (Japan) guidelines (2013). The data of linear regression analysis indicated a good linear relationship over the range of 0.16-0.64 μg/ml concentrations with correlation coefficient value of 0.993. The proposed method can be applied to the analysis of Etravirine from human plasma. Key Words: Etravirine, Human Plasma, HPLC, Bioanalytical study, Validation