ResearchBib Share Your Research, Maximize Your Social Impacts
Sign for Notice Everyday Sign up >> Login

PCR DETECTION OF STREPTOCOCCUS PNEUMONIAE DNA FROM NASOPHARYNX OF THE PATIENTS WITH ACUTE RESPIRATORY INFECTION

Journal: Indo American Journal of Pharmaceutical Sciences (IAJPS) (Vol.04, No. 06)

Publication Date:

Authors : ; ; ;

Page : 1762-1767

Keywords : PCR; Streptococcus pneumoniae; Ply; Nasopharynx;

Source : Downloadexternal Find it from : Google Scholarexternal

Abstract

Causing pneumonia, sepsis, meningitis, and otitis media often in young children and elderly adults, streptococcus pneumoniae is considered as not only an aggressive pathogen but a normal part of the human respiratory microbiome, as well. To identify S. pneumoniae, some methods which are mostly culture-based are usually used. In general, occasionally being validated by DNA-based methods, culture-based detection methods depend on optochin susceptibility, agglutination, and bile-solubility. In the current study, a total number of 93 nasopharyngeal samples were collected from patients with acute respiratory infection. Isolates of S. pneumoniae were confirmed by α-hemolysis of colonies appeared, optochin sensitivity, and bile solubility. The presence of ply gene was also discovered by PCR. 7 (7.53%) isolates among 93 nasopharyngeal samples were confirmed as S. pneumoniae by all of phenotypic tests, while the PCR assay revealed that 19 isolates (20.43%) were positive for virulence gene, ply. The present study found out the identification of Streptococcus pneumoniae should be based on phenotypic tests and at least a molecular method such as PCR. Keywords: PCR, Streptococcus pneumoniae, Ply, Nasopharynx

Last modified: 2017-07-13 00:34:30