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Effective Biosurfactants Production by Pseudomonas aeruginosa and its Efficacy on Different Oils

Journal: Journal of Advanced Laboratory Research In Biology (Vol.1, No. 1)

Publication Date:

Authors : ; ; ; ; ;

Page : 31-34

Keywords : Biosurfactant production; Pseudomonas aeruginosa; Rhamnolipid; Test efficacy;

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A rhamnolipid producing bacterium, Pseudomonas aeruginosa was isolated from contaminated soil with oily wastes. The Pseudomonas aeruginosa grown with glucose and corn oil as a carbon source produced bio-surfactant. This bio-surfactant was purified by procedures that included chloroform-ethanol extraction and 0.05M bicarbonate treatments. The active compound was identified as rhamnolipid by using thin layer chromatography. The emulsification activity of bio-surfactant, the coconut oil responded better than the olive oil, groundnut oil and sunflower oil and gave a maximum level of 1cm

Last modified: 2021-02-01 22:18:31