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Characterization of Chromobacterium violaceum Isolated from Spoiled Vegetables and Antibiogram of Violacein

Journal: Journal of Advanced Laboratory Research In Biology (Vol.2, No. 1)

Publication Date:

Authors : ; ; ;

Page : 18-22

Keywords : Chromobacterium violaceum; purple colony; violacein; antibiotic susceptibility;

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Abstract

Chromobacterium violaceum, a Gram negative, facultative anaerobic, non-sporing coccobacillus, producing a natural antibiotic called violacein was extracted from Chromobacterium violaceum MTCC 2656, collection of Chandigarh. The present study reports different types of microbial population along with Chromobacterium violaceum in 36 samples of spoiled vegetables. In the numerous microbial populations, three different strains (HMCCC 45, HMCCC 46 and HMCCC 47) were isolated and subjected to various morphological and biochemical tests. The results reveal that the three strains isolated, HMCCC 46 was similar to the standard except that HMCCC 46 colonies were dark violet in colour. The antibiotic sensitivity test of HMCCC 46 revealed that it was resistant to vancomycin, ampicillin, linezolid, and susceptible to colistin, oxacillin, gentamicin, norfloxacin, chloramphenicol, amikacin.

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Last modified: 2018-07-23 13:30:44