VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION AND QUANTIFICATION OF RESERPINE AND GALLIC ACID IN AN AYURVEDIC FORMULATIONJournal: Indian Drugs (Vol.54, No. 9)
Publication Date: 2017-09-28
Authors : Adhyapak A. A.; Jadhav A. P.;
Page : 86-88
Keywords : ;
A simple, precise, accurate and robust RP-HPLC method was developed and validated for simultaneous estimation of reserpine and gallic acid in an ayurvedic formulation. The two markers were resolved using a C-18 column make Hemochrom Instil using the mobile phase acetonitrile: water (pH 5.6 adjusted using orthophosphoric acid) in the ratio 75:25 V/V at a flow rate of 0.8 mL/min. Retention times of reserpine and gallic acid were 8.178 ± 0.2 minutes and 2.527 ± 0.2 minutes respectively, at 229 nm. The developed method was validated on the basis of ICH Q2 (R1) guidelines. The developed method was applied for quantification of markers in marketed formulation, Cardostab tablet.
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