THE COMMUNICATION OF RS (505151) WITH AMOUNT OF PCSK9 GENE EXPRESSION IN CARDIOVASCULAR DISEASE

Cardiovascular Disease has been proposed as a sickness with considerable complications and consequent disabilities in industrialized countries. One of the main causes of heart diseases is deposition of fat and LDLCh in blood vessels and overexpression of PCSK9. This study was designed to investigate PCSK9 gene expression level in patients compared to control groups as well as most prevalent polymorphism of E670G gene. In the current study, 40 patients with CHD (Coronary heart disease) and 20 non - CHD cases were studied. PCSK9 gene expression was evaluated by qPCR and E670G polymorphism (SNP) was determined by RFLP PCR. PCSK9 gene expression in 60 cases including patients and control groups, PCSK9 gene expression was reduced in most of the cases. 75% of samples were patients with A/G genotype; only 10% of patients were G/G genotype. Other samples were homozygous with A/A genotype. Evaluation of PCSK9 gene expression level and its associated SNP (E670G) in patients with cardiovascular disease can be effective in early diagnosis in order to reduce mortality rates of cardiovascular lesions and increase life expectancy. Key Words: CVD, Hypercholesterolemia, PCSK9, SNP.