EVALUATION OF ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITY OF SELECTED MEDICINAL PLANTS USING DIFFERENT MODELS IN SWISS ALBINO MICE BRAIN

The current research was aimed to estimate and compare the antioxidant effect of selected medicinal plants collected from different areas of Balochistan, Pakistan. The antioxidant activitywas determined by 2, 2-diphenyl-1- picryl hydrazyl(DPPH) a radical scavenging assay, lipid peroxidation assay and total antioxidant assay. Plant extracts demonstratedhangups alongside thiobarbituric acid reactive species (TBARS) tempted by pro-oxidant 10uM ferroussulphate (FeSO4) or sodium nitroprusside in the brain region of mice. Plants extracts showed significantferric reducing and free radical scavenging activity and also showed the higher concntration of both flavonoids and phenols. It was observed that higher thetotal polyphenolicand falavonoidscontentsbetter the antioxidant effect. All the plant samples showed the order of their antioxidant activity as SolanumNigram>Momordicacharantia>SolanumVillosum>TinosporaCordifolia> Ginkgo Biloba>Cuscutareflexa. The data obtained in the present study shows that all selected plants have inhibitory effect and antioxidant activity. The antioxidant effect of the selected plants might be related to the higher concentration of phenolic. Contents,ferric reducing ability and free radical scavenging activity.In this study we tried to provide the scientific proof for the traditional use of the selectedmedicinal plants as antioxidants. Key Words: Antioxidant activity, Free radical, DPPH, Medicinal plants, Oxidative stress, Mice brain.