QUALITATIVE AND QUANTITATIVE ANALYSIS OF PHENOLS AND FLAVONOIDS IN FOLIAR EXTRACT OF ANTHOCEPHALUS CADAMBA (ROXB.)

Cadamba is an important medicinal plant used to treat various diseases like fever, anemia, diabetes and many others. The present study deals with the determination of total phenol and flavonoids content by using Spectroscopy. Anthocephalus cadamba is a medium sized plant which belongs to the family of Rubiaceae and found everywhere in India. This plant takes six to eight years for flowering and grows upto six meter in hight. In the present study qualitative analysis was done to know the presence of various compounds where as the quantitative analysis was done to evaluate the concentration of them. Experiments were carried out for the phenol and flavanoids by using five solvents i.e petroleum ether, chloroform, ethanol, methanol and distilled water. Generally most of the phenolic compounds are linked to other compounds like proteins, cellulose, lignins and etc. Due to presence of these compounds phenols are important for the plant. Flavonoids are present in nature and have many biological activities such as anti-microbial, anti-inflamatory anti-tumor and many others. Result for phenol revealed that methanol extract contains higher phenol level. The concentration of phenol remains in between the range of 0.018128 - 0.559438 mgGA/g. It is lower in chloroform but higher in methanol. The order of the phenol content was chloroform < petroleum ether < distilled water < ethanol < methanol. Result for flavonoids shows that distilled water extract contains higher flavonoids content i.e 15.2 mg RU/g. The order of flavonoids content was distilled water> ethanol> methanol> chloroform> petroleum ether. Keywords: Quantitative analysis, Qualitative analysis, Anthocephalus cadamba, Phenols, Flavonoids.