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Peculiarities of Lungs Mass of Immature Rats by Inhalation of Toluene

Journal: Ukrainian journal of medicine, biology and sport (Vol.2, No. 2)

Publication Date:

Authors : ;

Page : 49-53

Keywords : lungs; mass; toluene; rats;

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In connection with the development of new technologies, the progress of science and technology is accompanied by the emergence of new synthetic materials. In the environment there is an emission of industrial wastes, which contain toxic volatile components, which is an environmental problem. This is evidenced by the analysis of samples of atmospheric air, soil and water in industrial areas in which an increased content of aromatic hydrocarbons was detected, including toluene in chemical structure. In these environmental conditions, the body reacts, and the respiratory reaction reacts first of all. Endocrine, immune, and central nervous system, there are metabolic disorders and the mechanisms of formation of an eco-dependent pathological process are launched. Infringements depend on concentration and duration of exposure of a toxic substance, a combination of it with other factors. The main sources of toluene in the environment are: epoxy resins, refining of petroleum products, exhaust gases, tobacco smoke. The contact of people with toluene occurs, as a rule, in the production associated with epoxy resins. The question of the effect of toluene on respiratory system organs remains topical, since the organs of the respiratory system are the most sensitive to the action of chemical factors. The experimental study was carried out on 60 white mongrel male rats aged 1 month from birth and the initial body weight was 40.83±3.58 g. The animals were divided into two groups. The first group included intact animals. The second group included animals that received daily inhalations of toluene with a single exposure for two months in an inhalation unit. Inhaled injection of toluene was simulated from 8 to 13.00 pm (5 hours daily) for 60 days. After sessions of inhalation exposure to epoxy vapor at 1, 7, 15, 30 and 60 days, the animals were decapitated from the experiment under ether anesthesia. Immediately after decapitation, the lungs were removed with a single complex with trachea, bronchi, mediastinal fiber, dissection was performed, and then weighed on an analytical balance of VLA-200 to within 1 mg. The analysis of digital data was carried out with the help of a computer program for organ and morphometric studies "Morpholog" ("Certificate of copyright registration " No. 9604 ", authors: VV Ovcharenko, VV Mavrich, 2004). The results of lung mass parameters were processed using statistical programs, the probability of error was less than 5% (p<0.05). There was a significant decrease in the weight of organs in accordance with control animals. As a consequence of the experiment, we found that after 60 days of inhalation exposure to the organism of toluene, the weight of the lungs decreases in comparison with the control during all periods of the readaptation period. The weight reduction in the right lung is more pronounced than in the left one. The maximum decrease occurs on the first day, by 60 days the decrease is less significant.

Last modified: 2017-11-04 17:52:30