Development and Validation of HPLC Method for Estimation of Etodolac in Rat Plasma

Etodolac is 1, 8-diethyl-1, 3, 4, 8- tetrahydropyrano [3,4-b] indole-1-acetic acid, used for osteoarthritis. This study was designed to develop and validate high performance liquid chromatography method of etodolac in rat plasma. The samples were analyzed by HiQ Sil C18 HS (250 × 4.6 mm, 5μ.) columns, using Acetonitrile: 0.02M potassium dihydrogen orthophosphate (65: 35 v/v) as a mobile phase. The method showed linearity (r2= 0.9964) over a concentration range of (1-25μg/ml). The method showed good mean recovery (97.53%) for etodolac. The method was found to be accurate, precise, linear, specific, sensitive and stable.