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Method for determination of amino acid content in protein products for medical use

Journal: Revista de Pielarie Incaltaminte / Leather and Footwear Journal (Vol.17, No. 4)

Publication Date:

Authors : ;

Page : 241-254

Keywords : collagen; amino acids; biomaterials;

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The paper presents a method for determination of amino acid content in collagen biomaterials for medical use produced in the Collagen Department of INCDTP - Division ICPI and its validation. The method has three stages: the first consists in hydrolysing the collagen biomaterial sample down to amino acids; the second step refers to derivatization of amino acids with N, O-bis (trimethylsilyl) trifl uoroacetamide (BSTFA) which is a silylation reagent; derivatized amino acids are detected using a mass spectrometer after gas chromatography. The method was validated to establish performance parameters and to check compliance with the intended purpose.

Last modified: 2017-12-20 19:27:33